Related Experiment Video

Updated: Dec 31, 2025

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
14:07

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Published on: November 23, 2012

16.9K

Complete Genome Sequence of Serratia marcescens Podophage Pila

Loraine Melbern1, Kathryn Broussard1, Russell Moreland1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|January 4, 2020
PubMed

Insights

We sequenced the genome of Serratia marcescens podophage Pila, a virus that infects bacteria. This provides insights into phage Pila

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Multidrug-resistant *Serratia marcescens* strains are a significant cause of hospital-acquired infections.
  • Bacteriophages, viruses that infect bacteria, are potential therapeutic agents against resistant pathogens.

Purpose of the Study:

  • To present the annotated genome sequence of *Serratia marcescens* podophage Pila.
  • To provide genomic data for understanding phage-host interactions and potential applications.

Main Methods:

  • Whole-genome sequencing of podophage Pila.
  • Bioinformatic analysis for gene prediction and annotation.
  • Comparative genomics with related bacteriophages.

Main Results:

  • The complete genome sequence of podophage Pila was determined to be 38,678 base pairs.
  • Fifty-one protein-coding genes were predicted within the Pila genome.
  • Direct terminal repeats of 148 base pairs were identified.

Conclusions:

  • The annotated genome of podophage Pila offers a valuable resource for studying *Serratia marcescens* phages.
  • Genomic information can facilitate the development of phage-based strategies to combat multidrug-resistant bacteria.

More Related Videos

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/4306:06

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

2.0K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.7K

Related Concept Videos

Bacterial Phylum Spirochaetes01:30

Bacterial Phylum Spirochaetes

Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased...
490
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
387