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Related Experiment Video

Updated: Dec 31, 2025

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Rapid and sensitive exosome detection with CRISPR/Cas12a.

Xianxian Zhao1, Wenqing Zhang1, Xiaopei Qiu1

  • 1Department of Clinical Laboratory, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Analytical and Bioanalytical Chemistry
|January 4, 2020
PubMed
Summary

A new method uses a CD63 aptamer and CRISPR/Cas12a system for sensitive exosome detection. This approach aids in disease diagnosis and prognosis, particularly for cancers like lung cancer.

Keywords:
AptasensorCD63CRISPR/Cas12aExosome

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanotechnology

Background:

  • Exosomes are implicated in disease pathogenesis, especially cancer.
  • Accurate and rapid exosome detection is crucial for disease diagnosis and prognosis.

Purpose of the Study:

  • To develop a sensitive and specific method for exosome detection.
  • To utilize the CD63 aptamer and CRISPR/Cas12a system for exosome quantification.

Main Methods:

  • Exosome detection based on CD63 aptamer recognition of exosomal membrane proteins.
  • Signal amplification using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system.
  • Quantification of exosomes within a range of 3 × 10^3 to 6 × 10^7 particles per microliter.

Main Results:

  • The method demonstrated high sensitivity and specificity in exosome detection.
  • Successful application in detecting exosomes in clinical samples from healthy individuals and lung cancer patients.
  • Results showed high consistency with nanoparticle tracking analysis.

Conclusions:

  • The developed method offers a highly sensitive and specific approach for exosome detection.
  • This technique provides a promising avenue for future exosome-based disease diagnosis.