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Quantitative high-performance thin-layer chromatography of dansyl derivatives of biogenic amines
1Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Analytical Biochemistry
|August 1, 1988
Abstract:
A procedure for the separation, detection, and quantification of picomole levels of dansyl derivatives of the biogenic amines, dopamine, norepinephrine, octopamine, and serotonin, has been developed using high-performance thin-layer chromatography. The detection limit is 1 to 2 pmol. Each of the amine derivatives has been detected in insect brain tissue and a solvent system has been developed for the separation and quantification of octopamine in insect tissue samples.