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Determination of nitrilotriacetate in biological matrices using ion exclusion chromatography
R P Schneider1, F Zürcher, T Egli
1Institute of Aquatic Sciences, Swiss Federal Institute of Technology, Zürich.
Abstract:
A sensitive method for the determination of nitrilotriacetate in biological growth media and cell-free extracts by ion exclusion chromatography is described using HCl as an eluant. The eluant conductivity was chemically suppressed with a membrane suppressor and a conductivity detector was used for subsequent detection. The membrane was continuously regenerated with a tetrabutylammoniumhydroxide solution. The detection limit for nitrilotriacetate in cell-free extract was 11 mg/liter, while for nitrilotriacetate in growth media it was 1 mg/liter. Interference by compounds present in biological matrices with the determination is discussed.