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Related Experiment Videos

Keratomycosis--a retrospective histopathologic and microbiologic analysis.

R Arora1, K Venkateswarlu, V M Mahajan

  • 1Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi.

Annals of Ophthalmology
|August 1, 1988
PubMed
Summary

Histopathology and microbiologic culture of corneal buttons show high fungal identification rates. Mixed infections, particularly with Staphylococcus epidermidis, presented diagnostic challenges.

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Area of Science:

  • Ophthalmology
  • Mycology
  • Pathology

Background:

  • Corneal infections require accurate diagnosis for effective treatment.
  • Histopathology and microbiologic culture are key diagnostic methods for ocular infections.

Purpose of the Study:

  • To compare the diagnostic yield of concurrent histopathologic and microbiologic examinations in corneal buttons.
  • To evaluate the prevalence and characteristics of fungal and bacterial keratitis.

Main Methods:

  • Retrospective analysis of 61 corneal buttons.
  • Concurrent histopathologic and microbiologic examinations were performed.
  • Fungal and bacterial identification and isolation were assessed.

Main Results:

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  • Histologic fungus identification was achieved in 77.04% of cases.
  • Microbiologic culture yielded fungal growth in 75.4% of cases.
  • Staphylococcus epidermidis was the most common isolate in mixed infections; Aspergillus was the most prevalent non-dematiaceous fungus. Dematiaceous fungi were rarely identified and potentially represented contaminants.

    • Conclusions:

    • Concurrent histopathology and microbiologic culture are valuable for diagnosing fungal keratitis.
    • Mixed infections pose diagnostic challenges.
    • Dematiaceous fungi in corneal buttons may often be contaminants.