Related Experiment Video
Updated: Dec 31, 2025
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
Cell number considerations for blastocyst transfer in younger patients
Zhiren Liu1, Mingting Jiang2, Linyun He1
1Center for Reproductive Medicine, Department of Obstetrics and Gynaecology, 900 Hospital of the Joint Logistics Team, Fuzhou, People's Republic of China.
Objective:
To investigate the role of the cell number at day 3 in blastocyst selection.
Design:
Observational, retrospective, single-center clinical study.
Patient(S):
In part 1, 1211 single vitrified-warmed blastocyst transfer (SVBT) cycles were identified and reviewed. All the cycles were conventional in vitro fertilization (IVF) cycles and the first embryo transfer cycles. Most of patients had a risk of ovarian hyperstimulation syndrome and were young. In part 2, 864 IVF-derived blastocysts from 292 infertile couples underwent trophectoderm (TE) biopsy for preimplantation genetic testing for aneuploidies (PGT-A).
Intervention(S):
No patient intervention.
Main Outcome Measure(S):
The first part was an analysis of the correlation between the cell number at day 3 and live birth rate (LBR) after SVBT, and the second part was an analysis of the correlation between the cell number at day 3 and euploid rate (ER) of blastocysts.
Result(S):
In part 1, after correcting for the effects of other confounders, the cell number at day 3 had no significant effect on the LBR (OR 1.001, 95% CI 0.938-1.068). In part 2, after correcting for the effects of other confounders, the cell number at day 3 had no significant effect on the ER (OR 0.960, 95% CI 0.866-1.063).
Conclusion(S):
When the vitrified-warmed blastocysts obtained by conventional IVF are transferred into young patients, the cell number at day 3 is not a strong predictor of the LBR. In addition, the cell number at day 3 is not a strong predictor of ER of IVF-derived blastocysts too.
