Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulated Protein Degradation

Regulated Protein Degradation

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

The Proteasome

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

The Proteasome

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

The Proteasome

The Proteasome

Proteins: From Genes to Degradation

Proteins: From Genes to Degradation

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dual E3 ligase recruitment by monovalent degraders for tunable SMARCA 2/4 degradation.

Nature chemical biology·2026

Same author

Disruption of the SAGA CORE triggers collateral degradation of KAT2A.

Nature communications·2026

Same author

Selective TRPV2 Antagonists Derived from the Natural Product Piperlongumine Inhibit Cancer Cell Migration and Metastasis.

ACS chemical biology·2026

Same author

Mapping and Rewiring Biology via Proximity Induction.

Accounts of chemical research·2026

Same author

High-throughput ligand diversification to discover chemical inducers of proximity.

Nature chemical biology·2026

Same author

Monovalent pseudo-natural products supercharge degradation of IDO1 by its native E3 KLHDC3.

Nature chemistry·2026

Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026

Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026

Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026

Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026

Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026

Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 31, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

Targeted protein degradation: current and future challenges.

Alexander Hanzl¹, Georg E Winter¹

¹CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.

Current Opinion in Chemical Biology

|January 6, 2020

Summary

This summary is machine-generated.

Targeted protein degradation offers a novel therapeutic strategy by reducing disease-related protein levels. This approach, distinct from traditional inhibition, holds promise for expanding drug targets and enhancing selectivity.

Keywords:

Chemical biology E3 ligase Molecular glues PROTACs Targeted protein degradation

More Related Videos

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

Related Experiment Videos

Last Updated: Dec 31, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

Area of Science:

Pharmacology
Biochemistry
Drug Discovery

Background:

Traditional drug development focuses on inhibiting protein activity.
Targeted protein degradation (TPD) aims to reduce overall protein levels.
TPD utilizes small molecules to induce protein ubiquitination and degradation.

Purpose of the Study:

To review the paradigm-shifting potential of targeted protein degradation.
To highlight the advantages of TPD over conventional inhibitors.
To summarize remaining challenges in realizing TPD's therapeutic potential.

Main Methods:

Review of existing literature on targeted protein degradation.
Analysis of mechanistic principles of TPD.
Discussion of limitations and future directions in TPD.

Main Results:

TPD expands the druggable proteome beyond traditional inhibitor capabilities.
TPD enables the development of highly selective therapeutic strategies.
Key challenges must be addressed for TPD to reach its full potential.

Conclusions:

Targeted protein degradation represents a significant advancement in drug discovery.
Overcoming current challenges is crucial for therapeutic application of TPD.
Pharmacologic modulation of protein degradation pathways offers promising therapeutic avenues.