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Updated: Dec 31, 2025
05:51
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
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Small molecular interaction-based fluorescence enhancement for second near-infrared imaging
Wang Zian1, Liu Yang1, Wang Peng2,3
1School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, PR China.
Nanomedicine (London, England)
|January 7, 2020
Summary
Researchers developed an efficient strategy to boost second near-infrared (NIR-II) fluorescence intensity using novel liposomes. This advancement enables enhanced *in vivo* imaging with lower doses and reduced exposure times.
Area of Science:
- Biomedical Imaging
- Nanotechnology
- Optical Spectroscopy
Background:
- Second near-infrared (NIR-II) fluorescence imaging offers deep tissue penetration and high resolution.
- Enhancing NIR-II fluorescence intensity is crucial for improving imaging sensitivity and reducing required probe doses.
- Current methods for boosting NIR-II signals often involve complex synthesis or limited applicability.
Purpose of the Study:
- To develop a novel strategy for significantly enhancing the fluorescence intensity of NIR-II probes.
- To create optimized liposomal formulations for improved NIR-II fluorescence.
- To evaluate the *in vivo* efficacy of the enhanced NIR-II liposomes for biomedical imaging applications.
Main Methods:
- Preparation of NIR-II liposomes using the thin film hydration method.
- Evaluation of fluorescence properties, including intensity and emission spectra.
- Assessment of *in vivo* performance using low-dose administration and irradiation for angiography.
Main Results:
- Indocyanine green-IR1061 liposomes demonstrated a fourfold increase in fluorescence intensity compared to IR1061 liposomes.
- Cationic indocyanine green-IR1061 liposomes achieved a tenfold enhancement in fluorescence intensity.
- Optimized liposomes enabled angiography with significantly lower probe doses and shorter irradiation times.
Conclusions:
- A new and effective method for enhancing NIR-II fluorescence intensity has been successfully developed.
- The enhanced liposomal formulations provide a promising platform for high-resolution, high signal-to-background ratio fluorescence imaging.
- This strategy holds potential for advancing *in vivo* diagnostic and research imaging techniques.
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