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CircNF1-419 improves the gut microbiome structure and function in AD-like mice
Chen Diling1, Qi Longkai1, Guo Yinrui1
1State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
This study reveals a connection between brain circular RNA (circRNA) and gut microbiome. Overexpressing circNF1-419 in mice impacts brain function and alters gut bacteria, highlighting the gut-brain axis.
Area of Science:
- Neuroscience
- Microbiology
- Genetics
Background:
- Pre-experiments indicated altered brain circRNA and gut microbiota in Alzheimer's Disease (AD)-like mice.
- circNF1-419 showed potential to enhance autophagy and ameliorate dementia in AD-like mice.
- A potential link between circRNA and the gut microbiome was hypothesized.
Purpose of the Study:
- To investigate the connection between circRNA and the gut microbiome.
- To identify the effects of overexpressed circNF1-419 on the gut microbiome and brain function.
Main Methods:
- Utilized an adeno-associated virus (AAV) animal system for overexpressing circNF1-419 in the brain.
- Analyzed changes in brain cholinergic system and gut microbiota composition.
- Assessed intestinal homeostasis, physiology, and gut microbiota trajectory in newborn mice.
Main Results:
- Overexpression of circNF1-419 influenced the brain's cholinergic system.
- Significant alterations in gut microbiota composition were observed, including changes in specific bacterial groups like *Candidatus Arthromitus* and *Lachnospiraceae*.
- Intestinal homeostasis, physiology, and the gut microbiota trajectory in newborn mice were affected.
Conclusions:
- Demonstrated a direct link between circRNA and the gut microbiome.
- Expanded the 'microbiome-transcriptome' linkage library.
- Provided new insights into the gut-brain axis and its regulation by circRNA.
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