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Updated: Dec 31, 2025
Inducement and Evaluation of a Murine Model of Experimental Myopia
Published on: January 22, 2019
Regulatory roles of differentially expressed MicroRNAs in metabolic processes in negative Lens-induced myopia Guinea
Dadong Guo1, Meihua Ding2, Xiaoli Song3
1Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong; Eye Institute of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan, 250002, China. dadonggene@163.com.
Background:
Myopia is one of the most common vision defects worldwide. microRNAs can regulate the target gene expression, influencing the development of diseases.
Results:
To investigate the alterations of microRNA profiling in negative lens-induced myopia (NLIM) guinea pigs and to explore the regulatory role of microRNAs in the occurrence and the development of myopia, we first established the NLIM guinea pig model after induction for 2 weeks. Further, we isolated sclera to purify total messenger RNA (mRNA) in both NLIM and NLIM fellow sclera. Using next generation sequencing technique and bioinformatics analysis, we identified the differentially expressed microRNAs in NLIM guinea pigs, performed the bioinformatics annotation for the differentially expressed microRNAs, and validated the expression of differentially expressed microRNAs. As a result, we successfully established an NLIM model in guinea pigs, identified 27 differentially expressed microRNAs in NLIM guinea pig sclera, including 10 upregulated and 17 downregulated microRNAs. The KEGG annotation showed the main signaling pathways were closely associated with PPAR signaling, pyruvate and propanoate metabolisms, and TGF-beta signaling pathways.
Conclusions:
Our findings indicate that the development of myopia is mainly involved in the disorder of metabolic processes in NLIM guinea pigs. The PPAR signaling, pyruvate and propanoate metabolism pathways may play roles in the development of myopia.
Insights
Myopia development involves altered microRNA profiles in guinea pig sclera. Metabolic pathways, including PPAR signaling, are implicated in myopia progression.
Area of Science:
- Ophthalmology
- Molecular Biology
- Genetics
Background:
- Myopia is a prevalent global vision defect.
- MicroRNAs (miRNAs) are key regulators of gene expression and disease development.
Purpose of the Study:
- Investigate microRNA expression changes in a guinea pig model of myopia.
- Explore the role of microRNAs in myopia development.
Main Methods:
- Established a negative lens-induced myopia (NLIM) guinea pig model.
- Utilized next-generation sequencing and bioinformatics to analyze scleral miRNA profiles.
- Validated differentially expressed miRNAs.
Main Results:
- Successfully created an NLIM guinea pig model.
- Identified 27 differentially expressed miRNAs (10 upregulated, 17 downregulated) in NLIM sclera.
- KEGG analysis highlighted PPAR signaling, pyruvate and propanoate metabolism, and TGF-beta signaling pathways.
Conclusions:
- Myopia development is linked to metabolic process dysregulation in NLIM guinea pigs.
- PPAR signaling and metabolic pathways are potentially involved in myopia pathogenesis.
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