Simultaneous quantification of multiple biomarkers on a self-calibrating microfluidic paper-based analytic device.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Point-of-care testing (POCT) requires sensitive, reliable, and easy-to-use diagnostic tools.
• Existing multiplex assays often face challenges with calibration and environmental interference.
• Microfluidic paper-based analytical devices (μPADs) offer a promising platform for low-cost diagnostics.

Purpose of the Study:

• To develop a novel μPAD platform for simultaneous detection of multiple analytes.
• To incorporate self-calibration capabilities to minimize environmental influences.
• To demonstrate the feasibility of the platform for clinically relevant analytes like glucose and lactate.

Main Methods:

• Fabrication of μPADs using wax printing on chromatographic paper.
• Design incorporating distinct detection and calibrant zones with controlled wax barriers.
• Proof-of-concept demonstration using glucose and lactate detection.
• Analysis of results using naked-eye observation and flatbed scanner.

Main Results:

• Successful simultaneous detection of glucose and lactate from a small sample volume (0.4 μL).
• Achieved low detection limits: 0.3125 mM for glucose and 0.2975 mM for lactate.
• Demonstrated effective self-calibration, reducing environmental condition impact.
• Results were visually distinguishable and quantifiable via scanner.

Conclusions:

• The developed multiplex paper-based device is a cost-effective, user-friendly, and highly sensitive solution for point-of-care testing.
• The integrated self-calibration function enhances the reliability of the assay.
• This platform shows significant potential for clinical multianalyte diagnostics.