The sensitive "Turn-on" fluorescence platform of ascorbic acid based on conjugated polymer nanoparticles
Qiu-Bo Wang1, Cui-Jiao Zhang1, Hao Yu1
1School of Materials Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China; Shandong Provincial Key Laboratory of Processing and Testing Technology of Glass and Functional Ceramics, Key Laboratory of Amorphous and Polycrystalline Materials, Qilu University of Technology, Jinan, 250353, China.
Analytica Chimica Acta
|January 9, 2020
Summary
A new fluorescence platform using conjugated polymer nanoparticles (CPNs) offers a simple, sensitive, and selective method for detecting ascorbic acid (AA). This advanced technique achieves a low limit of detection and shows promise for analyzing AA in real-world samples.
Area of Science:
- Analytical Chemistry
- Materials Science
- Nanotechnology
Background:
- Ascorbic acid (AA) is vital for physiological functions, necessitating accurate quantification in food and pharmaceuticals.
- Existing methods for AA determination often suffer from low sensitivity, complexity, and high costs.
- Conjugated polymer nanoparticles (CPNs) offer promising optical properties, biocompatibility, and stability for sensing applications.
Purpose of the Study:
- To develop a simple, rapid, and highly sensitive fluorescence platform for quantitative ascorbic acid (AA) detection.
- To overcome the limitations of current AA determination methods.
- To explore the potential of CPNs in developing advanced analytical tools.
Main Methods:
- Preparation of a CPNsPBOC-COOH fluorescence platform using Poly [3-{2,5-bis (2-ethyl-hexyloxy)]-phenyl}-vinyl}-9-octyl-carbazole] (PBOC) and Polystyrene-maleic anhydride (PSMA).
- Quantitative detection of AA using the fluorescence platform.
- Investigation of logic gate control of CPN fluorescence by Fe3+ and AA.
Main Results:
- The CPNsPBOC-COOH platform demonstrated high selectivity and sensitivity towards AA.
- A linear relationship was observed between AA concentration (0.57–17.10 μM) and fluorescence intensity.
- A low limit of detection (LOD) of 10 nM was achieved, surpassing existing methods.
- The fluorescence of CPNsPBOC-COOH could be modulated by logic gates using Fe3+ and AA.
Conclusions:
- The developed CPNsPBOC-COOH fluorescence platform provides a sensitive and selective method for AA detection.
- The platform exhibits a low LOD, making it suitable for trace analysis.
- The successful application in real samples (vitamin C tablets, serum, plasma) confirms the platform's feasibility and practical utility.
