Intracellular metabolism of the interferon mediator, 2-5A, using permeabilized cells

2-5A synthetase and 2'-phosphodiesterase, the enzymatic activities which respectively synthesize and degrade the interferon mediator 2-5A (ppp(A2'p)nA), were studied in digitonin-permeabilized cells. 2-5A synthetase was higher in permeabilized than in lysed Daudi cells. Mouse L cells appeared to contain two different 2-5A synthetase activities, one of which could be separated from 2'-phosphodiesterase activity, which was only cytosolic. Permeabilization techniques offer opportunities to investigate (2',5')-oligoadenylate intracellular metabolism, which remains incompletely known.