Cell-Membrane-Based Biomimetic Systems with Bioorthogonal Functionalities.

Area of Science:

• Biomaterials Science
• Chemical Biology
• Nanotechnology

Background:

• Cell-based biomimetic systems offer unique advantages like prolonged circulation and immune evasion.
• Precise functionalization remains a significant challenge hindering their widespread application.
• Bioorthogonal chemistry provides a powerful tool for specific molecular modifications under physiological conditions.

Purpose of the Study:

• To develop a reliable strategy for cell membrane functionalization using bioorthogonal chemistry.
• To integrate bioorthogonal chemistry with biomimetic approaches for enhanced system performance.
• To explore the application of these engineered systems in various biomedical contexts.

Main Methods:

• Metabolic incorporation of unnatural precursors bearing bioorthogonal groups into living cells.
• Functionalization of cell membranes via bioorthogonal ligation reactions.
• Fabrication and application of engineered cell-based biomimetic systems.

Main Results:

• Demonstrated successful metabolic incorporation of bioorthogonal groups into cell membranes.
• Achieved precise anchoring of functional molecules through bioorthogonal chemistry.
• Developed versatile biomimetic systems for virus labeling, targeted drug delivery, and theranostics.

Conclusions:

• Bioorthogonal chemistry offers a safe, direct, and long-term strategy for engineering cell-based biomimetic systems.
• These engineered systems exhibit enhanced capabilities for labeling, targeting, imaging, and therapy.
• The integration of bioorthogonal chemistry and biomimetic systems holds significant promise for future biomedical applications and lab-to-clinic translation.