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Area of Science:

  • Biomaterials Science
  • Cryobiology
  • Polymer Chemistry

Background:

  • Cryopreservation requires slowing ice growth to prevent cell injury.
  • Conventional cryoprotective agents (CPAs) can be toxic for clinical applications.
  • Trehalose offers unique properties for cell stabilization but trehalose-based CPAs are understudied.

Purpose of the Study:

  • To synthesize and evaluate a novel trehalose-based polymer and hydrogel.
  • To assess its efficacy as a cryoprotectant and 3D cell scaffold.
  • To explore its potential in cell encapsulation and organoid development.

Main Methods:

  • Synthesis of trehalose-based polymers (poly(Tre-ECH)) and hydrogels.
  • In vitro cytotoxicity studies.
  • Cryopreservation of skin fibroblast, HeLa, and PC3 cell lines using controlled-rate and ultrarapid freezing.
  • Differential scanning calorimetry and splat cooling assays for ice recrystallization inhibition.
  • Assessment of post-thaw cell membrane integrity and plating efficiency.

Main Results:

  • Trehalose-based polymers demonstrated excellent biocompatibility up to 100 mg/mL.
  • High post-thaw cell membrane integrity and plating efficiencies were observed.
  • The polymers exhibited significant ice recrystallization inhibition activity.
  • Hydrogel formation capability was confirmed for 3D cell scaffolding.

Conclusions:

  • Novel trehalose-based polymers and hydrogels are effective and biocompatible cryoprotectants.
  • These materials show potential for cell organoid development and advanced cryopreservation platforms.
  • This work addresses the need for safer and more effective cryoprotective agents.