Bioactive bacterial cellulose membrane with prolonged release of chlorhexidine for dental medical application

Bioabsorbable barrier membrane is desired in dental medicine for treatment of periodontal diseases caused by different types of bacteria. Bioactive and bioabsorbable bacterial cellulose (BC) is a promising material for such application. However, a key challenge to implement this approach is produce BC membranes selectively oxidized and loaded with a bactericide, in order to modulate bioabsortion time and bactericide effect, respectively. In the present study, the drug model chlorhexidine (CHX) was chosen and NaIO₄ was used as oxidizing agent. To modulate CHX release and efficacy, inclusion complexes of CHX with β-cyclodextrin (CHX:βCD) were synthesized. A linear dependence between degree of oxidation (DO) and oxidant concentration was found (DO = 2.07 + 45 [NaIO₄]). CHX has strong chemical interaction with cellulose structure, contributing for its significant retention. The association of membrane oxidation and formation of the inclusion complex with βCD causes a 10-fold increase in CHX release rate compared to unmodified cellulose. Thus, validating the concept that CHX release can be modulated using these two strategies. All membranes loaded with CHX inhibited S. aureus, E. coli and C. albicans growth, but DABC+CHX:βCD showed greater inhibition zone (p < 0.05). That, associated with other results, indicates potential application as bioactive and bioabsorbable membrane.