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Updated: Dec 31, 2025
Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG
Published on: December 28, 2014
Real-time imaging of infarction deterioration after ischemic stroke in rats using electrical impedance tomography
Lu Cao1, Haoting Li, Danchen Fu
1Lu Cao and Haoting Li contributed equally to this work.
Objective:
This study investigated the feasibility of electrical impedance tomography (EIT) for monitoring the deterioration of ischemic lesion after the onset of stroke.
Approach:
Fifteen rats were randomly distributed into two groups: rats operated to establish a right middle cerebral artery occlusion (MCAO) (n = 10), and sham-operated rats (n = 5). Then, the operated rats were kept 2 h under anesthesia for EIT monitoring. Subsequently, descriptive statistical analysis was performed on whole-brain resistivity changes, and repeated-measures analysis of variance (ANOVA) on the average resistivity variation index. Additionally, pathological examinations were performed after 6 h of infarction.
Main Results:
The results obtained showed that ischemic damage developed in the right corpus striatum of the rats with MCAO, whereas the brains of the sham group showed no anomalies. The descriptive statistical analysis revealed that the whole-brain resistivity changes after 30, 60, 90, and 120 min of infarction were 0.063 ± 0.038, 0.097 ± 0.046, 0.141 ± 0.062, and 0.204 ± 0.092 for the rats with MCAO and 0.029 ± 0.021, 0.002 ± 0.002, 0.017 ± 0.011, and -0.001 ± 0.011 for the sham-operated rats, respectively. The repeated-measures ANOVA revealed that the right MCAO model resulted in a significant impedance increase in the right hemisphere, which continued to increase over time after infarction.
Significance:
The overall study results indicate that EIT facilitates monitoring of local impedance variations caused by MCAO and may be a solution for real-time monitoring of intracranial pathological changes in ischemic stroke patients.
