Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phospholipase activity in human bile.

T Nakano1, J Yanagisawa, F Nakayama

  • 1Department of Surgery I, Kyushu University Faculty of Medicine, Fukuoka, Japan.

Hepatology (Baltimore, Md.)
|November 1, 1988
PubMed
Summary

Bacterial infection significantly increases free fatty acids in bile, primarily due to bacterial phospholipase A1 activity. This suggests a key role for bacteria in forming brown pigment gallstones.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CHIP buffers heterogeneous Bcl-2 expression levels to prevent augmentation of anticancer drug-resistant cell population.

Oncogene·2014
Same author

Video-assisted thoracic surgery for lung cancer in hemodialysis patients.

Asian journal of endoscopic surgery·2012
Same author

Enhanced silicon oxidation on titanium-covered Si(001).

Journal of physics. Condensed matter : an Institute of Physics journal·2011
Same author

Less maintenance immunosuppression in lung transplantation following hematopoietic stem cell transplantation from the same living donor.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2011
Same author

KLF4 suppresses estrogen-dependent breast cancer growth by inhibiting the transcriptional activity of ERalpha.

Oncogene·2009
Same author

Role of catalase in monocytic differentiation of U937 cells by TPA: hydrogen peroxide as a second messenger.

Leukemia·2008

Area of Science:

  • Gastroenterology
  • Microbiology
  • Biochemistry

Background:

  • Brown pigment gallstones are a common ailment.
  • The role of bacterial infection in gallstone formation is not fully understood.
  • Free fatty acids (FFAs) are implicated in gallstone pathogenesis.

Purpose of the Study:

  • To investigate the role of bacterial infection in the formation of free fatty acids (FFAs) in hepatic bile.
  • To determine the contribution of bacterial phospholipase activity to FFA accumulation.
  • To elucidate the specific phospholipase enzymes involved in brown pigment gallstone formation.

Main Methods:

  • Comparison of FFA concentrations and phospholipase activity in hepatic bile from infected and non-infected sources.
  • Quantification of biliary phospholipase activity using a radiolabeled substrate ([14C]dipalmitoyl phosphatidylcholine).
  • Identification of bacterial strains from bile and analysis of their phospholipase profiles (A1 and A2).

Main Results:

  • Bile with bacterial infection showed significantly higher concentrations of FFAs compared to non-infected bile.
  • Biliary phospholipase activity was significantly elevated in the presence of bacterial infection.
  • A strong positive correlation was observed between biliary phospholipase activity and FFA concentration.
  • Bacterial strains isolated from bile exhibited both phospholipase A1 and A2 activity, while human sources predominantly showed A2 activity.

Conclusions:

  • Bacterial infection is a significant factor in increasing free fatty acid levels in bile.
  • Bacterial phospholipase A1 activity appears crucial for cleaving palmitic acid from phosphatidylcholine, contributing to calcium palmitate formation in brown pigment gallstones.
  • These findings highlight the importance of bacterial enzymes in the pathogenesis of brown pigment gallstones.

Related Experiment Videos