UV-Visible spectroscopic effect on Haemoglobin & DNA degradation: A forensic approach

In forensic casework, sample degradation is a largely an unsolved problem that exists due to poor preservation protocols. In Indian subcontinent, the forensic blood stains are commonly exposed to different ecological conditions for months before they are collected and transferred to the forensic laboratory for analysis. Under such conditions, the question of haemoglobin (Hb) & nucleic acid stability is of utmost importance. The biological evidence, especially the blood sample that underwent extreme conditions should further be retained in an appropriate storage facility until processed for the forensic serology/DNA testing. The present study provides the spectroscopic approach of analyzing Hb and DNA degradation of forensic blood samples preserved under varied conditions (tabulated and graphically). These samples were further subjected to and DNA analysis to assess the quality of sample based on different preservation conditions. At last, this study suggests the effective and appropriate preservation protocol of sample soon after its collection.