THE IMPACT OF HYDROXYAPATITE SINTERING TEMPERATURES ON THE STRUCTURAL AND FUNCTIONAL CAPACITY OF EMBRYONAL LUNG
Hamed Benghuzzi1, Michelle Tucci1, Ibrahim Farah2
1University of Mississippi Medical Center, Jackson, MS.
Biomedical Sciences Instrumentation
|January 14, 2020
Summary
Hydroxyapatite (HA) sintered at 1200°C promoted Embryonal Lung Fibroblast (MRC-5) proliferation and maintained cell morphology. Higher sintering temperatures (700-1300°C) caused cellular damage and fragmentation, indicating sintering temperature is crucial for HA biomaterials.
Area of Science:
- Biomaterials Science
- Cell Biology
- Materials Engineering
Background:
- Hydroxyapatite (HA) is a key biomaterial for bone regeneration due to its similarity to bone mineral.
- Optimizing HA properties, such as density and mechanical strength, is crucial for effective tissue repair.
- Cellular response to biomaterials is highly dependent on their physical and chemical characteristics.
Purpose of the Study:
- To investigate the impact of hydroxyapatite (HA) sintered at varying temperatures (700, 1000, 1200, 1300°C) on Embryonal Lung Fibroblast (MRC-5) cell proliferation, morphology, and viability.
- To determine the optimal sintering temperature for HA to support healthy cell growth and function.
- To assess cellular membrane integrity and morphological changes induced by different HA sintering temperatures.
Main Methods:
- Preparation of HA microcrystals and sintering at 700, 1000, 1200, and 1300°C.
- Fabrication of HA disks using L-lysine as a binder and cold-pressing.
- Culturing MRC-5 fibroblasts with HA samples and assessing proliferation, morphology, and Malondialdehyde (MDA) levels via TBARS assay.
Main Results:
- HA sintered at 700, 1000, and 1300°C initially increased cell proliferation (24-48 hours), but caused cellular membrane damage and fragmentation by 72 hours.
- HA sintered at 1200°C showed no significant difference in proliferation at 24-48 hours but led to a 2-3 fold increase in cell number by 72 hours, without causing cellular damage or morphological changes.
- Calculated density of HA devices was directly proportional to sintering temperature.
Conclusions:
- Sintering temperature is a critical factor in developing hydroxyapatite (HA) delivery systems with desirable mechanical properties and biocompatibility.
- HA sintered at 1200°C supports optimal fibroblast proliferation and morphology, suggesting its potential for tissue engineering applications.
- Achieving optimal HA density through controlled sintering is essential for developing effective material constructs for tissue defect repair.
