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Single-cell ATP detection and content analyses in electrostimulus-induced apoptosis using functionalized glass
Dandan Wang1, Guohua Qi2, Ya Zhou1
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. ydjin@ciac.ac.cn and University of Science and Technology of China, Hefei, Anhui 230026, China.
Abstract:
A glass nanopipette functionalized with ATP-responsive gold nanoparticle assemblies was developed for ATP detection in single-cells and used for analysing the content change of ATP during electrostimulus (ES)-induced apoptosis. The variation of ATP content in single normal cells and cancer cells during apoptosis was detected by the method.
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