Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Top-Down Mass Spectrometry-Based Structural and Functional Proteomics: A Perspective.

Journal of mass spectrometry : JMS·2026
Same author

Intact Proteoform Analysis by Capillary Electrophoresis-Mass Spectrometry. Are We There Yet?

Angewandte Chemie (International ed. in English)·2025
Same author

Methionine Oxidation Footprinting in Intact Proteins (MOFIP) Using Top-Down Proteomics.

Proteomics·2025
Same author

Top-Down Thermal Proteome Profiling (TD-TPP) for Functional Characterization of the Intact Proteoforms in Complex Samples.

Journal of mass spectrometry : JMS·2025
Same author

High-Throughput Top-Down Proteomic Analysis of Picogram-Level Complex Samples Using Multisegment Spray-Capillary CE-MS.

Analytical chemistry·2025
Same author

Establishment of multiple machine learning prognostic model for gene differences between primary tumors and lymph nodes in luminal breast cancer.

Breast cancer research and treatment·2024
Same journal

Metabolite-centric identification of antimetabolite drug targets across cancer and neurodegenerative diseases.

Molecular omics·2026
Same journal

Platelet proteomics on less than a drop of previously frozen, non-citrate plasma.

Molecular omics·2026
Same journal

Decoding the diabetic transition: a lipidomic approach for biomarker discovery in an Indian cohort.

Molecular omics·2026
Same journal

Placental metabolomics for assessment of pregnancy complications: a systematic review.

Molecular omics·2026
Same journal

Prostate cancer: metabolic remodelling in expressed prostatic secretions reveals cellular structural changes measured by mpMRI.

Molecular omics·2026
Same journal

A practical guide to experimental design and power analysis for metaproteomics studies.

Molecular omics·2026
See all related articles

Related Experiment Video

Updated: Dec 31, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.9K

High-throughput quantitative top-down proteomics.

Kellye A Cupp-Sutton1, Si Wu

  • 1Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Room 2210, Norman, OK 73019-5251, USA. si.wu@ou.edu.

Molecular Omics
|January 15, 2020
PubMed
Summary
This summary is machine-generated.

Quantitative top-down mass spectrometry (MS) advances proteoform analysis for understanding protein modifications. This review covers methods, applications, and future directions for quantitative top-down MS in proteomics.

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.5K
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

5.0K

Related Experiment Videos

Last Updated: Dec 31, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.9K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.5K
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

5.0K

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Top-down mass spectrometry (MS) enables intact protein analysis at the proteoform level, crucial for understanding protein modification functions.
  • Advancements in high-resolution MS, liquid chromatography (LC), and data analysis have boosted the popularity and capability of top-down proteomics.
  • Distinguishing unique proteoforms via top-down MS has revealed significant variations in biological functions.

Purpose of the Study:

  • To review quantitative top-down MS methods for intact protein identification.
  • To discuss current applications of quantitative top-down MS in studying proteoform-specific biological functions.
  • To present emerging areas for future implementation of quantitative top-down MS analysis.

Main Methods:

  • Review of established and emerging top-down mass spectrometry techniques.
  • Analysis of liquid chromatography (LC) separation efficiencies for intact proteins.
  • Evaluation of data analysis software for quantitative proteoform identification.

Main Results:

  • Qualitative identification of proteoforms is often insufficient for determining biological relevance.
  • Quantitative top-down MS enables exploration of proteomes at the proteoform level, revealing functional differences.
  • Significant variations in biological function exist between distinct protein proteoforms.

Conclusions:

  • Quantitative top-down MS is essential for elucidating the biological significance of protein proteoforms.
  • The field is rapidly advancing, offering new possibilities for in-depth proteome analysis.
  • Further development and application of quantitative top-down MS will enhance our understanding of complex biological systems.