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Updated: Dec 31, 2025

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
Published on: November 4, 2016
Primary T-Cell Transduction to Study Follicular Helper T-Cell Differentiation
Yang Zhang1, Xuehui Long1, Xiaoming Wang2
1Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu, China.
Abstract:
Follicular helper T (Tfh) cells constitute a specialized CD4+ T-cell subset that localizes in close proximity to B cells and are essential in the production of high-affinity, class-switched antibodies, and their dysregulations are also involved in autoimmune diseases. Modulating gene expression patterns in primary T cells is an important approach to understanding Tfh cell differentiation and function. In this chapter, we describe a protocol to evaluate Tfh cell differentiation with OT-II TCR transgenic T cells by retrovirally transducing gene of interest. This protocol adopts the recombinant retrovirus-based transduction of primary CD4+ T cells, and it also includes procedures for adoptive transfer, immunization, and flow cytometry analysis.
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