Related Experiment Video

Updated: Dec 30, 2025

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

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A novel regulatory function for miR-217 targetedly suppressing fibronectin expression in keloid fibrogenesis

Wei Wu1, Feng Xie1, Yi Zhang2

  • 1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.

International Journal of Clinical and Experimental Pathology
|January 16, 2020
PubMed

Insights

MicroRNA-217 (miRNA-217) suppresses fibronectin (FN) expression, inhibiting keloid scarring fibroblast growth. This finding suggests miRNA-217 as a potential therapeutic target for keloid fibrogenesis.

Area of Science:

  • Molecular Biology
  • Dermatology
  • Biochemistry

Background:

  • Fibronectin (FN) is crucial in keloid scarring (KS) development.
  • MicroRNAs (miRNAs) are implicated in post-translational gene regulation.
  • The role of specific miRNAs in FN regulation within keloid fibroblasts (KSFBs) requires elucidation.

Purpose of the Study:

  • To investigate the regulatory role of miRNA-217 on fibronectin (FN) expression in keloid scarring fibroblasts (KSFBs).
  • To explore the potential of miRNA-217 as a therapeutic target for keloid fibrogenesis.

Main Methods:

  • Analysis of miRNA-217 and FN expression in clinical KS tissues and adjacent normal tissues.
  • Bioinformatic prediction of miRNA-217 targets using PicTar, TargetScan, and miRBase.
  • In vitro experiments involving miRNA-217 gain-of-function and loss-of-function in KSFBs.
  • Assessment of FN, Collagen-1 (Col-1), and Collagen-3 (Col-3) protein levels.

Main Results:

  • miRNA-217 expression was significantly suppressed in KS tissues, inversely correlating with elevated FN mRNA levels.
  • miRNA-217 directly targeted FN, impacting KSFB proliferation and apoptosis.
  • Overexpression of miRNA-217 reduced FN, Col-1, and Col-3 protein levels, while its inhibition increased them.

Conclusions:

  • miRNA-217 suppresses KSFB growth, primarily by inhibiting FN expression.
  • miRNA-217 demonstrates potential as a therapeutic strategy for mitigating keloid fibrogenesis.
Keywords:
MiRNA-217fibroblastsfibronectinkeloid fibrogenesis

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