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Analysis of Circulating HMGB1 in Human Serum
Weiqiang Chen1, Guoqiang Bao1, Lin Zhao1
1Laboratory of Emergency Medicine, The Feinstein Institute for Medical Research, Manhasset, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 16, 2020
Summary
High-mobility group box 1 (HMGB1) is a nuclear protein involved in inflammation and cancer. This study introduces a new method to measure HMGB1 in human serum, comparing it to existing ELISA techniques.
Area of Science:
- Biochemistry
- Immunology
- Oncology
Background:
- High-mobility group box 1 (HMGB1) is a nuclear protein with roles in inflammation and cancer.
- HMGB1 can be released from damaged cells or secreted by immune cells, acting as a mediator in inflammatory diseases.
- Serum HMGB1 levels are implicated as a biomarker for cancer diagnosis and therapy, influenced by treatments like chemotherapy and radiation.
Purpose of the Study:
- To describe a novel semiquantitative immune blotting method for measuring HMGB1 in human serum.
- To compare the performance of this new method with a commercially available HMGB1 enzyme-linked immunosorbent assay (ELISA).
Main Methods:
- Development and application of a semiquantitative immune blotting technique.
- Measurement of HMGB1 concentrations in human serum samples.
- Comparative analysis against a standard HMGB1 ELISA kit.
Main Results:
- The study details a new semiquantitative immune blotting assay for serum HMGB1.
- Performance comparison data between the immune blotting method and ELISA are presented.
Conclusions:
- A novel semiquantitative immune blotting method for serum HMGB1 measurement is established.
- This method offers an alternative for assessing HMGB1 levels, potentially aiding in cancer biomarker research.
