Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

807
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
807

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications.

New biotechnologyĀ·2025
Same author

Oleoyl coenzyme A triggers peroxygenase activity in cytochrome c.

Journal of inorganic biochemistryĀ·2025
Same author

Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses.

SLAS technologyĀ·2023
Same author

Hepatic adenomatosis in a young woman with non-familial maturity-onset diabetes of the young type 3.

Canadian liver journalĀ·2022
Same author

Polymer-Protein Core-Shell Nanoparticles for Enhanced Antigen Immunogenicity.

ACS macro lettersĀ·2022
Same author

Purification, Characterization, and Structural Studies of a Sulfatase from <i>Pedobacter yulinensis</i>.

Molecules (Basel, Switzerland)Ā·2022

Related Experiment Video

Updated: Dec 30, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

25.0K

Parallel sample processing using dispersive INtip micro-purification on programmable multichannel pipettes.

Patrick A Kates1, John J Tomashek1, David A Miles1

  • 1Integrated Micro-Chromatography Systems, Inc., Irmo, SC, USA.

Biotechniques
|January 16, 2020
PubMed
Summary
This summary is machine-generated.

Researchers can now automate biomolecule purification using innovative pipette tip dispersive solid-phase extraction (INtip). This method enhances throughput for high-throughput biomolecular workflows, overcoming limitations of manual techniques.

Keywords:
INtip chemistryautomationchromatographydispersive solid-phase extractionhigh-throughput screeningpurification

More Related Videos

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.2K
A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.6K

Related Experiment Videos

Last Updated: Dec 30, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

25.0K
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.2K
A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

11.6K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Current biomacromolecule separation methods require manual intervention, hindering high-throughput automation.
  • Manual processes limit the scale and efficiency of biomolecular sample processing.
  • Developing automated separation techniques is crucial for advancing research.

Purpose of the Study:

  • To characterize biomacromolecule affinity purification using dispersive solid-phase extraction in a pipette tip (INtip).
  • To evaluate the efficiency, kinetics, and binding capacity of INtip technology.
  • To assess the scalability of INtip for automated platforms.

Main Methods:

  • Utilized commercially available resin for INtip purification.
  • Compared INtip efficiency against traditional batch and spin column methods.
  • Measured binding kinetics and resin binding capacities.
  • Tested INtip on an automated platform (INTEGRA ASSIST).

Main Results:

  • INtip technology effectively purifies biomacromolecules.
  • Efficiency was comparable to established methods, with potential for higher throughput.
  • Binding kinetics and capacities were characterized.
  • INtip demonstrated scalability and effectiveness on an automated system.

Conclusions:

  • INtip represents a novel, automatable method for biomacromolecule purification.
  • This technology overcomes manual intervention limitations in current separation techniques.
  • Integration of INtip into automated platforms will significantly benefit high-throughput biomolecular workflows.