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Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats
Lízia Daniela E Silva Nascimento1, Renata Amadei Nicolau2, Antônio Luiz Martins Maia Filho3
1Fellow PhD degree, Postgraduate Program in Biomedical Engineering, Universidade do Vale do Paraíba (UNIVAP), Sao Jose dos Campos-SP. Assistant Professor of Kinesiology, MSc, Health Sciences Center, Physical Therapy, Universidade Estadual do Piauí (UESPI), Teresina-PI, Brazil. Conception, design, intellectual and scientific content of the study; acquisition and interpretation of data; technical procedures; manuscript preparation.
Purpose:
To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair.
Methods:
Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically.
Results:
Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107).
Conclusion:
Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.
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