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A Reversible Rhodamine B Based pH Probe with Large Pseudo-Stokes Shift.

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This study introduces DPE-Rh, a novel pH probe utilizing Förster resonance energy transfer (FRET). It offers sensitive and reversible pH detection with a significant fluorescence enhancement in the acidic range.

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Area of Science:

  • Chemical sensing
  • Fluorescent probes
  • Molecular spectroscopy

Background:

  • Förster resonance energy transfer (FRET) is a key mechanism in fluorescent sensing.
  • Developing sensitive and selective probes for environmental monitoring is crucial.
  • Rhodamine B derivatives are widely used in fluorescent probe design.

Purpose of the Study:

  • To develop a reversible and sensitive pH probe based on FRET.
  • To investigate the sensing mechanism and performance of the DPE-Rh probe.
  • To demonstrate the probe's tolerance to metal ions and its ability to minimize excitation light interference.

Main Methods:

  • Förster resonance energy transfer (FRET) between 1,2-diphenylethyne (DPE) and Rhodamine B (Rh).
  • Spectroscopic analysis of fluorescence intensity changes in response to pH variations.
  • 1H NMR spectrometry to elucidate the sensing mechanism.

Main Results:

  • The DPE-Rh probe exhibited a reversible and sensitive response to pH.
  • A ca. 1000-fold fluorescence enhancement was observed with a linear response over pH 2.0–5.5.
  • The FRET mechanism resulted in a 240 nm excitation-emission shift and 100% donor emission quenching.
  • The probe demonstrated tolerance to various metal ions.

Conclusions:

  • DPE-Rh serves as an effective FRET-based fluorescent probe for sensitive pH detection.
  • The probe design offers a strategy to mitigate interference from excitation light.
  • The study highlights the potential of FRET systems for developing advanced chemical sensors.