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Updated: Dec 30, 2025
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
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Cyclin E1 expression and malignancy in meningiomas.
Benedito Jamilson Araújo Pereira1, Pedro Augustto de Santana Júnior1, Antonio Nogueira de Almeida2
1Laboratório de Biologia Molecular e celular (LIM 15), Departmento de Neurologia, Faculdade de Medicina FMUSP, Universidade de São Paulo, SP, Brazil.
Clinical Neurology and Neurosurgery
|January 17, 2020
Summary
Cyclin E1 (CCNE1) gene expression predicts meningioma malignancy. The fibrous subtype of grade I meningiomas shows the highest CCNE1, SKP2, and P27 gene expression levels.
Area of Science:
- Neuro-oncology
- Molecular biology
- Cancer research
Background:
- Meningiomas are tumors arising from the meninges.
- Accurate grading and identification of tumor progression markers are crucial for effective treatment.
- The Skp2-p27-cyclin E1 pathway is implicated in cell cycle regulation and cancer progression.
Purpose of the Study:
- To investigate the Skp2-p27-cyclin E1 pathway as a potential tumor progression marker in meningiomas.
- To correlate gene expression levels with meningioma grades and histological subtypes.
Main Methods:
- Quantitative real-time PCR was used to measure gene expression levels of CCNE1, SKP2, and P27.
- Expression levels were compared across meningioma grades (I-III) and within histological subtypes of grade I meningiomas.
Main Results:
- CCNE1 expression was significantly higher in grade II meningiomas compared to grade I, and it reliably predicted grade II tumors.
- CCNE1 expression correlated with SKP2 and P27 expression in grade I meningiomas.
- The fibrous subtype of grade I meningiomas exhibited the highest CCNE1, SKP2, and P27 gene expression. Higher cyclin E1 protein was found in atypical meningioma nuclei.
Conclusions:
- CCNE1 expression serves as a predictive marker for meningioma malignancy.
- The fibrous subtype of grade I meningiomas demonstrates elevated gene expression within this pathway.
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