Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.4K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.4K
Activation of Integrins01:15

Activation of Integrins

4.8K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
4.8K
Integrins01:10

Integrins

5.1K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
5.1K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

3.4K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
3.4K
Anchoring Junctions01:03

Anchoring Junctions

4.7K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
4.7K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

8.7K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monocytes and neutrophils promote cardiac fibroblast pro-fibrotic phenotypes through IL-6 and MIF.

Frontiers in cell and developmental biology·2026
Same author

Data modeling the interplay between single-cell shape, single-cell protein expression, and tissue state.

Cell reports methods·2026
Same author

Building capacity in imaging data management.

Journal of microscopy·2026
Same author

Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts.

Communications chemistry·2025
Same author

High content 3D imaging by dual-view oblique plane microscopy.

PNAS nexus·2025
Same author

Ligand and integrin-independent mechano-sensitive EGFR activation in lung cancer cells.

Journal of cell science·2025

Related Experiment Video

Updated: Dec 30, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.7K

New perspectives on integrin-dependent adhesions.

Magdalene Michael1, Maddy Parsons1

  • 1Randall Centre for Cell and Molecular Biophysics, King's College London, New Hunts House, Guys Cam, London, SE1 1UL, UK.

Current Opinion in Cell Biology
|January 17, 2020
PubMed
Summary
This summary is machine-generated.

Integrins, crucial cell adhesion receptors, are better understood thanks to new research. Advances reveal complex molecular interactions and physical controls governing integrin signaling pathways.

Keywords:
AdhesionAdhesion dynamicsCytoskeletonIntegrinsKindlinMicroscopyMigrationTalin

More Related Videos

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

8.8K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.2K

Related Experiment Videos

Last Updated: Dec 30, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.7K
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

8.8K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.2K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Biophysics

Background:

  • Integrins are heterodimeric transmembrane receptors.
  • They link the extracellular matrix to the actin cytoskeleton.
  • Integrins assemble various adhesion structures via adaptor molecules.

Purpose of the Study:

  • To deepen the understanding of integrin signaling.
  • To explore associated regulatory processes.
  • To investigate the molecular and physical control of integrin signaling.

Main Methods:

  • Biochemical methods
  • Imaging techniques
  • Biophysical methods

Main Results:

  • Recent advances have significantly improved understanding of integrin signaling.
  • The identification of integrin-based 'adhesomes' has defined core components of adhesion complexes.
  • Unexpected protein behaviors and recruited molecules have expanded knowledge of integrin signaling control.

Conclusions:

  • New insights into integrin signaling have emerged.
  • Adhesomes provide a framework for understanding adhesion complexes.
  • Molecular and physical factors play critical roles in controlling integrin signaling.