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Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

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Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
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Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy
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Decoding with Calcium Signals from Layer 2/3 Motor Cortex during A Pressing Movement.

Ruixue Wang, Jiawei Han, Jing Chen

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |January 18, 2020
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    Summary

    Optical imaging using one-photon microscopy shows potential for brain-machine interfaces (BMIs). Calcium signals from motor cortex neurons reveal distinct activity patterns, crucial for accurate neural decoding in BMI applications.

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    Area of Science:

    • Neuroscience
    • Biomedical Engineering
    • Optical Imaging

    Background:

    • Traditional electrophysiological recordings in brain-machine interfaces (BMIs) offer sparse spatial data and lack neuron-type information.
    • Optical imaging methods, such as calcium imaging, provide dense, spatially organized neural data with potential for genetic annotation.

    Purpose of the Study:

    • To demonstrate the potential of calcium imaging signals from a one-photon microscope for neural decoding in BMIs.
    • To analyze neural activity at single-neuron and population levels during a learned motor task.

    Main Methods:

    • Calcium signals were recorded from layer 2/3 motor cortex neurons in mice trained on a lever-press task using a one-photon microscope.
    • Neural activity was analyzed at both individual neuron and population levels.
    • Decoding algorithms were applied to assess movement-related information.

    Main Results:

    • Two distinct classes of pressing-related neurons were identified.
    • Unique neuronal population activity patterns were observed during pressing movements compared to baseline.
    • Decoding accuracy was significantly improved by utilizing population response structures.

    Conclusions:

    • Calcium imaging with one-photon microscopy is a viable method for neural decoding in BMIs.
    • Accurate decoding in BMIs relies on neural signals from a larger number and diverse types of neurons.
    • Population activity structure is key for specifying movement-related information in neural decoding.