Changes of bladder mucosal inflammatory factors and prognosis in cystitis glandularis

Objective: To investigate the relationships of bladder mucosal inflammatory factors, interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) with the occurrence and development of cystitis glandularis (CG), and their effects on the prognosis. Methods: A total of 61 patients with CG from January 2010 to 2014 were randomly selected. Tissue specimens of postoperative patients were collected. 16 cases of normal bladder mucosa during the same period were collected as a control group. Blood specimens and fresh tissue specimens were collected from 6 patients with CG. The messenger ribonucleic acid (mRNA) levels of IL-1, IL-6 and TNF-α were detected via reverse transcription polymerase chain reaction (RT-PCR). The protein levels of IL-1, IL-6 and TNF-α in serum of patients with GC and normal controls were detected via an enzyme-linked immunosorbent assay (ELISA). The protein expressions of IL-1, IL-6 and TNF-α were detected via immunohistochemistry (IHC), and their relationships with the clinical features and prognosis of GC were analyzed. A Cox proportional hazard regression model was used for multivariate analysis on the prognostic factors of CG, and all the tests were performed with 95% confidence interval (CI). Results: The protein expressions of IL-1, IL-6 and TNF-α in patients with CG were obviously higher than those in normal group. The mRNA levels of IL-1, IL-6 and TNF-α in the serum of patients with CG were also significantly higher than those in normal group (P<0.05). The expressions of IL-1, IL-6 and TNF-α in CG were positively correlated. TNF-α is an independent prognostic factor of CG. Conclusion: IL-1, IL-6 and TNF-α are associated with the occurrence and development of CG. TNF-α presents as an independent prognostic factor of CG that can be used for the diagnosis of cystitis glandularis.