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Updated: Dec 30, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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hsa_circ0021347 as a Potential Target Regulated by B7-H3 in Modulating the Malignant Characteristics of Osteosarcoma

Ling Wang1,2, Guo-Chuan Zhang2, Fu-Biao Kang3

  • 1Department of Orthopedics, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Biomed Research International
|January 18, 2020
PubMed

Insights

This study reveals that hsa_circ0021347 is downregulated in osteosarcoma (OS) and linked to better patient survival. Its expression is negatively correlated with B7-H3, suggesting a role in OS progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • B7-H3 is implicated in osteosarcoma (OS) development and immune evasion.
  • The role of B7-H3 deficiency and associated circRNA alterations in OS progression remains unclear.

Purpose of the Study:

  • Investigate the impact of B7-H3 silencing on circRNA expression in OS.
  • Identify and validate circRNAs associated with OS progression and B7-H3 expression.
  • Explore the clinical significance and potential mechanisms of identified circRNAs in OS.

Main Methods:

  • Stable B7-H3 silencing in OS cells.
  • Validation using western blotting and real-time PCR.
  • CircRNA array analysis for differential expression.
  • Correlation analysis with clinicopathological features.
  • Bioinformatic pathway analyses (GO, KEGG, PANTHER).

Main Results:

  • hsa_circ0021347 was significantly downregulated in OS tissues and cell lines.
  • hsa_circ0021347 expression showed a negative correlation with B7-H3 levels in OS.
  • hsa_circ0021347 downregulation was associated with advanced Enneking stage and poorer patient survival.
  • Predicted miRNA interaction network for hsa_circ0021347.

Conclusions:

  • hsa_circ0021347 may function as a tumor suppressor in osteosarcoma.
  • hsa_circ0021347 is a potential diagnostic and prognostic biomarker for OS.
  • Understanding the hsa_circ0021347/miRNA network could inform novel therapeutic strategies for OS.

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