Analyzing Metabolomics Data for Environmental Health and Exposome Research

The exposome is the cumulative measure of environmental influences and associated biological responses across the life span, with critical relevance for understanding how exposures can impact human health. Metabolomics analysis of biological samples offers unique advantages for examining the exposome. Simultaneous analysis of external exposures, biological responses, and host susceptibility at a systems level can help establish links between external exposures and health outcomes. As metabolomics technologies continue to evolve for the study of the exposome, metabolomics ultimately will help provide valuable insights for exposure risk assessment, and disease prevention and management. Here, we discuss recent advances in metabolomics, and describe data processing protocols that can enable analysis of the exposome. This chapter focuses on using liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics for analysis of the exposome, including (1) preprocessing of untargeted metabolomics data, (2) identification of exposure chemicals and their metabolites, and (3) methods to establish associations between exposures and diseases.