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This study introduces a novel bubble array method using horseshoe structures to simultaneously trap and rotate cells in microfluidic devices. This technique enables precise cell manipulation for 3D morphology reconstruction and parameter calculation.

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Area of Science:

  • Microfluidics
  • Acoustic Streaming
  • Cell Biology

Background:

  • Acoustic streaming vortices generated by bubbles in microfluidic chambers can manipulate bio-samples.
  • Current methods require optimization for efficient cell capture and rotation.

Purpose of the Study:

  • To propose and validate a bubble array strategy using horseshoe structures for simultaneous cell capture and rotation.
  • To investigate the impact of geometric parameters on bubble homogeneity and cell trapping efficiency.
  • To demonstrate the application of cell rotation for 3D morphology reconstruction.

Main Methods:

  • Configured a bubble array in regularly arranged horseshoe structures within a microfluidic chamber.
  • Applied travelling surface acoustic wave oscillation in the ultrasonic range.
  • Modified geometric parameters of horseshoe structures and microfluidic settings.
  • Validated simulation and experimental results of bubble-induced streaming vortices.
  • Achieved in-plane and out-of-plane rotation of arrayed HeLa cells.

Main Results:

  • Achieved high bubble homogeneity and cell trapping percentage by optimizing geometric parameters.
  • Confirmed consistency between simulation and experimental results for bubble-induced streaming vortices.
  • Successfully demonstrated both in-plane and out-of-plane rotation of trapped HeLa cells.
  • Utilized out-of-plane rotation for accurate 3D cell morphology reconstruction and parameter calculation.

Conclusions:

  • The proposed bubble array method effectively captures and rotates cells in microfluidic devices.
  • This technique offers a promising tool for investigating bioengineering, biophysics, medicine, and cell biology.
  • The ability to reconstruct 3D cell morphology is valuable for calculating cell geometry-related parameters.