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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
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Developing front-end devices for improved sample preparation in MS-based proteome analysis.

Alan A Doucette1, Jessica L Nickerson1

  • 1Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.

Journal of Mass Spectrometry : JMS
|January 21, 2020
PubMed
Summary
This summary is machine-generated.

Developing new scientific instruments, particularly front-end devices for mass spectrometry proteomics, can significantly enhance research. This guide outlines the iterative device design process, from concept to testing, to inspire future innovation.

Keywords:
injection moldinginstrument designmass spectrometryproteomicssample preparation

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Proteomics

Background:

  • Chemical analysis relies heavily on instrumentation for precision measurements.
  • Novel instrument development offers a valuable approach to solving scientific challenges.
  • Existing proteomics workflows can be augmented by improved front-end sample manipulation devices.

Purpose of the Study:

  • To outline the comprehensive process of novel device design, from conception to manufacturing and testing.
  • To highlight the development of advanced front-end devices for protein sample preparation in mass spectrometry.
  • To inspire instrument design by sharing insights into the iterative development process, including challenges and choices.

Main Methods:

  • Conceptualization and design of new instrumental tools.
  • Manufacturing and rigorous testing of developed devices.
  • Focus on front-end innovations for protein sample manipulation prior to mass spectrometry analysis.

Main Results:

  • A detailed perspective on the iterative device design process is presented.
  • Specific attention is given to enhancing proteomics workflows through improved sample handling.
  • Key considerations, challenges, and training aspects inherent in instrument innovation are discussed.

Conclusions:

  • Instrument design is a critical pathway for addressing scientific problems and advancing research.
  • Improved front-end devices can significantly augment proteomics workflows and mass spectrometry applications.
  • A summary of points to consider is provided to encourage and guide future instrument innovation.