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Optimizing the u411 automated urinalysis instrument for veterinary use.
Samantha J M Evans1, Julia L Sharp2, Linda M Vap1
1Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Science, Colorado State University, Fort Collins, CO, USA.
Veterinary Clinical Pathology
|January 21, 2020
Summary
Optimizing the Cobas u411 Analyzer with reflectance programming adjustments significantly improved canine and feline urinalysis accuracy. This enhances efficiency and consistency in veterinary diagnostic labs.
Area of Science:
- Veterinary Diagnostics
- Clinical Pathology
- Analytical Chemistry
Background:
- The Cobas u411 Analyzer is an automated, reflectance photometry instrument for human urinalysis.
- It utilizes Roche's CHEMSTRIP 10UA technology and is designed for human urine samples.
Purpose of the Study:
- To optimize and validate the Cobas u411 Analyzer for canine and feline urinalysis.
- To assess the instrument's performance in veterinary clinical pathology settings.
Main Methods:
- Compared Cobas u411 results with manual readings for canine and feline urine samples.
- Adjusted instrument reflectance programming based on initial comparisons and bias evaluation.
- Validated adjusted settings with additional samples and generated ROC curves for protein.
Main Results:
- Adjustments increased concordance by up to 17.7% for protein, 11.7% for ketones, and 4.5% for bilirubin.
- Discordance for all analytes remained low (≤3%).
- Canine and feline samples showed similar discordance levels, with slight advantages for dogs in some analytes.
Conclusions:
- Refined Cobas u411 reflectance programming enhances concordance for canine and feline urinalysis.
- The validated instrument offers potential for increased efficiency and consistency in high-throughput veterinary labs.
