Ochrobactrum soli sp. nov., Isolated from a Korean Cattle Farm

A Gram stain negative, motile, non-spore-forming, rod-shaped, strictly aerobic, beige-pigmented bacterium, designated strain BO-7^T, was isolated from soil of cattle farm, in Seosan, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain BO-7^T clustered with species of the genus Ochrobactrum and appeared closely related to O. haematophilum CCUG 38531^T (98.9%), O. daejeonense KCTC 22458^T (98.1%), O. rhizosphaerae DSM 19824^T (98.1%), O. pituitosum DSM 22207^T (98.0%), and O. pecoris DSM 23868^T (98.0%). The digital DNA-DNA hybridization and average nucleotide identity between strain BO-7^T and the closely related strains were 21.9-39.1%, 78.5-89.5%, respectively, indicating that BO-7^T is a novel species of the genus Ochrobactrum. The DNA G + C content of the genomic DNA was 57.1 mol%, and ubiquinone Q-10 was the predominant respiratory quinone. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethyl-ethanolamine, di-phosphatidylglycerol, the major polyamines were spermidine, putrescine, and sym-homospermidine. The major cellular fatty acids (> 5%) were C_16:0, C_19:0 cycle ω7c, and C_18:1ω7c and/or C_18:1ω6c (summed feature 8). ANI calculation, digital DNA-DNA hybridization, physiological and biochemical characteristics indicated that strain BO-7^T represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum soli sp. nov. is proposed. The type strain is BO-7^T (= KACC 19676^T = LMG 30809^T).