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A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers
Imee M A Del Mundo1, Eun Jeong Cho, Kevin N Dalby
1Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX, USA. karen.vasquez@austin.utexas.edu.
Abstract:
Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA ligands was demonstrated using BePI and doxorubicin. Identification of triplex-interacting ligands may allow the regulation of genetic instability in human genomes.
