In Situ Growth of Covalent Organic Framework Shells on Silica Microspheres for Application in Liquid Chromatography
Lu-Liang Wang1, Cheng-Xiong Yang1, Xiu-Ping Yan1,2
1College of Chemistry, Research Center for Analytical Sciences, Tianjin Key Laboratory of Molecular Recognition and Biosensing, State Key Laboratory of Medicinal Chemical Biology, Nankai University, 94 Weijin Road, Tianjin, 300071, China.
Abstract:
A facile, in situ growth strategy for the synthesis of monodispersed COF@SiO2 microspheres is reported. The microspheres have uniform and controllable COF shells, and are used as the stationary phase for HPLC. The TpBD COF, constructed from 1,3,5-triformylphloroglucinol (Tp) and benzidine (BD), is taken as the COF shell, and aminosilica (SiO2 -NH2 ) is employed as the core to support the TpBD shell. The TpBD shell thickness is adjusted by controlling the concentrations of the Tp and BD monomers. The TpBD@SiO2 packed columns show excellent hydrophobic selectivity, and good reproducibility for the separation of neutral (toluene and ethylbenzene, polycyclic aromatic hydrocarbons), acidic (hydroquinone, p-cresol, and p-chlorophenol), and basic molecules (nucleobases, nucleosides, and deoxynucleosides).
Related Concept Videos
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Ion-Exchange Chromatography
Diffusion on Chromatography Columns
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation


