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Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology.

Chen Zhu1, Xi Luo1,2, Wilfred Villariza Espulgar1

  • 1Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Micromachines
|January 23, 2020
PubMed
Summary

This study introduces a novel biosensor for detecting Interleukin-6 (IL-6) at the single-cell level. The device enables real-time monitoring of cytokine secretion, offering insights into cell viability and biological variations.

Keywords:
Interleukin 6 (IL-6) detectionlocalized surface plasmon resonance (LSPR) technologysingle cell level immunoassaysingle cell trapping

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Cytokine secretion analysis is crucial for clinical diagnostics.
  • Localized surface plasmon resonance (LSPR) biosensors offer label-free, real-time biomolecule monitoring.
  • Existing LSPR sensors often lack single-cell resolution and can be complex to fabricate.

Purpose of the Study:

  • To develop a simple, cost-effective biosensor for single-cell Interleukin-6 (IL-6) detection.
  • To enable real-time monitoring of cytokine secretion from individual cells.
  • To overcome limitations of traditional LSPR sensors in single-cell analysis.

Main Methods:

  • Synergistic integration of microwell chip for cell trapping and gold-capped nanopillar cyclo-olefin-polymer (COP) film.
  • Utilizing localized surface plasmon resonance (LSPR) peak red-shift in the transmittance spectrum for detection.
  • In-situ analysis of cytokine secretion from individually trapped cells.

Main Results:

  • Successful detection of IL-6 at the single-cell level.
  • Demonstration of real-time monitoring of cytokine secretion.
  • The biosensor provides a simple and efficient platform for analyzing cellular responses.

Conclusions:

  • The developed biosensor enables precise, single-cell level analysis of IL-6.
  • This technology facilitates real-time monitoring of cellular cytokine secretion.
  • The device holds potential for assessing cell viability and biological variations in single cells.