Characterization of a novel herbicide and antibiotic-resistant Chlorella sp. with an extensive extracellular matrix

A herbicide and antibiotic-resistant microalgal strain, isolated from a eutrophic site at Giofyros river (Heraklion, Crete, Greece) was extensively characterized. In the presence of relatively high concentrations of common photosynthesis inhibitors (DCMU and atrazine), as well as various antibiotics (spectinomycin, kanamycin, and chloramphenicol), the green microalga was able to increase its biomass in approximately equal levels compared to the control. Despite the high concentrations of the inhibitors, photosynthetic efficiency and chlorophyll a amount per dry cell biomass were comparable to those of control cultures in almost all cases. 18S rDNA analysis showed that this microalga belongs to the Chlorella genus. Optical and electron microscopy studies revealed the presence of an extensive extracellular matrix (EM) that surrounds the cells and plays an important role in colony formation and cell-cell interactions. Fourier transform infrared spectroscopy provided evidence that the EM consists of a polysaccharide. This matrix could be separated from the cells with a simple centrifugation. Depending on growth conditions, the dry cell biomass of this Chlorella strain was found to contain 35-39% proteins and 27-42% carbohydrates. The results of this study have demonstrated that the EM plays a protective role for cell homeostasis maintenance against the various chemical agents. This green microalga is a suitable candidate for further studies regarding sustainable biomass production in waste waters for a series of applications.