Direct evidence using a controlled greenhouse study for threshold effects of soil organic matter on crop growth

Soil organic matter (SOM) is a key indicator of soil fertility, and building SOM is assumed to decrease reliance on external inputs and ensure stable crop production. Recent syntheses of field data support this assumption with positive SOM-productivity relationships that asymptote at ~4% SOM. Teasing out the directionality of this relationship-the extent to which SOM increases crop growth vs. greater growth leading to higher SOM concentrations-requires controlled experimentation. To disentangle this causative pathway, we conducted a greenhouse experiment whereby we manipulated SOM concentrations from 1% to 9% and evaluated whether the SOM-productivity relationship differed for spring wheat (Triticum aestivum, L.) under nitrogen fertilization crossed with irrigation due to the expectation that SOM buffers the effects of reduced fertilization and/or irrigation. We found that higher concentrations of SOM led to greater productivity (measured as aboveground biomass) up to a threshold of 5% SOM, after which productivity declined across all treatments. These declines occurred despite the fact that indicators of soil health (water-holding capacity, microbial biomass, and bulk density) improved linearly with increasing SOM concentrations. That is, improvements in soil properties did not translate to gains in productivity at the highest SOM levels. Nitrogen fertilization led to greater productivity across all treatments, but to a greater relative extent at lower SOM levels, where we found that productivity on unfertilized soils with 4% SOM matched that of fertilized soils with 2% SOM. Differences in productivity on unfertilized soils due to irrigation emerged at higher SOM levels (>5%), highlighting SOM's role in water retention. Our results demonstrate that building SOM leads to improved growth of a globally important crop; however, our results also indicated a pronounced SOM threshold, after which crop growth declined. This underscores the need to develop optimal SOM targets for desired agricultural and environmental outcomes.