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Counting-based microfluidic paper-based devices capable of analyzing submicroliter sample volumes.

Md Almostasim Mahmud1, Eric J M Blondeel2, Brendan D MacDonald1

  • 1Faculty of Engineering and Applied Science, Ontario Tech University (UOIT), 2000 Simcoe Street North, Oshawa, Ontario L1G 0C5, Canada.

Biomicrofluidics
|January 23, 2020
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Summary

Researchers developed novel microfluidic paper-based analytical devices (µPADs) for semiquantitative testing using minimal sample volumes. This counting-based lateral flow assay (LFA) approach enables user-friendly analysis with finger-prick blood samples.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic paper-based analytical devices (µPADs) offer advantages like low cost and ease of use.
  • Analyzing small sample volumes (submicroliter) presents challenges for accurate semiquantitative readouts.
  • Existing methods often require trained personnel and larger sample volumes.

Purpose of the Study:

  • To develop semiquantitative counting-based lateral flow assay (LFA)-type µPADs for submicroliter sample analysis.
  • To overcome the limitations of interpreting color intensity in traditional assays.
  • To enable user-friendly, low-cost diagnostics using minimal sample volumes.

Main Methods:

  • Development of LFA-type µPADs utilizing a counting-based approach.
  • Implementation of a flow strategy with a running liquid to facilitate assay progression.
  • Validation using glucose and total human immunoglobulin E (IgE) tests with 0.5 µL and 1 µL samples, respectively.

Main Results:

  • Successful semiquantitative analysis of glucose (0-12 mmol/L) and IgE (0-400 ng/mL) using submicroliter volumes.
  • Demonstration of a counting-based readout correlating dot count to analyte concentration.
  • Confirmation of the first counting-based LFA-type µPADs for semiquantitative testing with minimal sample volumes.

Conclusions:

  • The developed counting-based LFA-type µPADs provide a user-friendly and accurate method for semiquantitative analysis of biological samples.
  • Submicroliter sample volumes are feasible for diagnostics, enhancing multiplexing and reducing costs.
  • This technology holds potential for point-of-care diagnostics and applications requiring minimal sample collection.