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Circulating exosomes and exosomal lncRNA HIF1A-AS1 in atherosclerosis

Yingge Wang1,2,3,4,5,6, Jingyan Liang1,2,3,4,5, Jiang Xu2,6

  • 1Department of Anatomy, Medical College, Yangzhou University Yangzhou, China.

International Journal of Clinical and Experimental Pathology
|January 23, 2020
PubMed

Insights

Exosomes and exosomal long non-coding RNA HIF1A-AS1 show promise as diagnostic biomarkers for atherosclerosis. Elevated levels of both were found in patients, indicating their potential for early disease detection.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Exosomes mediate intercellular communication and transfer long non-coding RNAs (lncRNAs).
  • lncRNA HIF1A-AS1, transferable via exosomes, is implicated in atherosclerosis pathogenesis.
  • This study investigates the diagnostic utility of exosomes and exosomal lncRNA HIF1A-AS1 in atherosclerosis.

Purpose of the Study:

  • To evaluate the diagnostic accuracy of exosome concentration and exosomal lncRNA HIF1A-AS1 expression in atherosclerosis patients.
  • To determine the correlation between exosome concentration and lncRNA HIF1A-AS1 levels in atherosclerosis.

Main Methods:

  • Plasma samples from 35 atherosclerosis patients and 28 healthy controls were analyzed.
  • Exosomes were isolated and quantified using flow cytometry.
  • Exosomal RNA was extracted, and lncRNA HIF1A-AS1 expression was measured via qRT-PCR.

Main Results:

  • Exosome concentration and exosomal lncRNA HIF1A-AS1 expression were significantly higher in atherosclerosis patients compared to healthy controls.
  • A positive correlation was observed between exosome concentration and lncRNA HIF1A-AS1 expression.
  • Receiver operating characteristic (ROC) curve analysis yielded area values of 0.856 for exosomes and 0.823 for lncRNA HIF1A-AS1.

Conclusions:

  • Exosomes and exosomal lncRNA HIF1A-AS1 demonstrate potential as novel biomarkers for atherosclerosis diagnosis.
  • These findings suggest a role for exosomal components in atherosclerosis detection and potentially in disease mechanisms.
Abstract

Keywords:
Exosomal lncRNAHIF1A-AS1atherosclerosisbiomarker

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