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Nuclear reprogramming in nuclear transplant rabbit embryos.

S L Stice1, J M Robl

  • 1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003.

Biology of Reproduction
|October 1, 1988
PubMed
Summary
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Researchers successfully produced the first six genetically verified nuclear transplant rabbits using eight-cell stage blastomere nuclear transfer. This breakthrough demonstrates successful nuclear reprogramming in mammalian embryo multiplication.

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Genetics

Background:

  • Nuclear transplantation is a key technique in assisted reproduction.
  • Mammalian cloning efficiency remains a challenge, particularly in rabbits.

Purpose of the Study:

  • To establish a reliable method for producing genetically verified nuclear transplant rabbits.
  • To investigate the efficiency and reprogramming capacity of nuclear transfer in rabbit embryos.

Main Methods:

  • Nuclear transfer of individual eight-cell stage blastomeres into enucleated mature oocytes.
  • Assessment of nuclear transplantation efficiency rates (chromosomal removal, fusion, activation, transfer).
  • Analysis of blastocyst cell number and morphology in nuclear transplant embryos.

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Main Results:

  • Six full-term, genetically verified nuclear transplant rabbits were produced from 164 manipulated eggs.
  • Efficiency rates for key steps were determined: chromosomal removal (92%), fusion (84%), activation (46%), embryo transfer (27%).
  • Nuclear transplant blastocysts showed reduced cell numbers and delayed formation, indicating successful nuclear reprogramming.

Conclusions:

  • Mature oocytes can reprogram eight-cell stage nuclei, enabling successful development to term in rabbits.
  • Low efficiency may be linked to oocyte aging and asynchronous transfers.
  • Successful nuclear reprogramming opens possibilities for expanded multiplication of mammalian embryos via serial nuclear transfer.