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Updated: Dec 30, 2025
Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Determining acrosomal status of the cynomolgus monkey (Macaca fascicularis) sperm by fluorescence microscopy
Nicholas L Cross1, Patricio Morales1, Masaru Fukuda1
1Division of Reproductive Biology and Medicine, Department of Obstetrics and Gynecology, University of California, Davis.
Abstract:
The acrosome of Macaca fascicularis sperm cannot be distinguished by conventional light microscopy, so determining whether sperm are acrosome-intact or-reacted is difficult. We describe methods for labeling the acrosomal region of sperm with two different probes: fluoresceinated Pisum sativum agglutinin and anti-sperm antiserum. Acrosome-intact sperm are much more heavily labeled in the acrosomal region than are acrosome-reacted sperm, providing a simple means of differentiating the two types of sperm. The two probes detect similar numbers of acrosome-reacted sperm following treatment with the divalent cation ionophore, A23187.
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