Rapid and Sensitive Drug Quantification in Tissue Sections Using Matrix Assisted Laser Desorption Ionization-Ion
Margaux Fresnais1,2, Alexander Muck3, Marius Majewsky1
1Department of Clinical Pharmacology and Pharmacoepidemiology, Heidelberg University Hospital, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Journal of the American Society for Mass Spectrometry
|January 24, 2020
Summary
Ion mobility spectrometry (IMS) enables rapid drug quantification in tissue sections. This technique effectively separates drugs from biological backgrounds, proving crucial for pharmacological MALDI imaging analysis.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biophysics
Background:
- Ion mobility spectrometry (IMS) offers rapid mass spectrometric separation for complex samples.
- IMS excels at separating isobaric compounds where traditional methods fail, such as in MALDI imaging.
- Pharmacological analysis of drugs in tissue sections presents significant analytical challenges.
Purpose of the Study:
- To demonstrate the utility of IMS for on-surface drug quantification in tissue sections using MALDI.
- To validate IMS as a method for discriminating drugs from complex biological matrices.
- To establish a proof-of-concept for IMS in pharmacological tissue analysis.
Main Methods:
- Utilized Ion Mobility Spectrometry (IMS) coupled with MALDI.
- Employed a profiling approach for sample preparation.
- Quantified the anthelmintic drug mebendazole (MBZ) as a model compound.
Main Results:
- Successfully discriminated mebendazole (MBZ) from the biological background in tissue sections.
- Achieved quantification of MBZ in the range of 5 to 5,000 ng/g.
- Established a limit of detection of 1 ng/g of tissue within 2 hours.
Conclusions:
- IMS is a valuable separation technique for on-surface drug quantification in tissue sections.
- IMS enhances the analysis of low-concentration drugs in complex biological samples.
- This study validates IMS for pharmacological MALDI imaging applications.
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