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Updated: Dec 30, 2025

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Published on: May 6, 2020
Identification of Cellular MicroRNA Coupling Strand Displacement Polymerization and Nicking-Endonuclease-Based
Peng Miao1,2, Yuguo Tang1,2, Qi Zhang3
1CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, P. R. China.
Abstract:
A target induced recycling amplification method for the detection of cellular microRNA is reported based on strand displacement polymerization and nicking endonuclease mediated cleavage. Moreover, by utilizing silver nanoparticle-based solid-state Ag/AgCl reaction, ultrahigh sensitivity is achieved with powerful discriminating towards the identification of target microRNA. The limit of detection is as low as 70 am. This method also allows the detection of cellular microRNA by lysing cells, thus making it a powerful tool for microRNA expression profiling and disease diagnosis.
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