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Symplasmic isolation marks cell fate changes during somatic embryogenesis.

Kamila Godel-Jedrychowska1, Katarzyna Kulinska-Lukaszek1, Anneke Horstman2,3

  • 1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.

Journal of Experimental Botany
|January 25, 2020
PubMed
Summary
This summary is machine-generated.

Callose deposition at plasmodesmata isolates plant cells, establishing totipotency for somatic embryo development. This process is crucial for controlling cell communication and development in Arabidopsis.

Keywords:
BABY BOOMWOX2Arabidopsisauxinplasmodesmataplasmodesmata size exclusion limitsomatic embryogenesissymplasmic communicationsymplasmic domain

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Cell-to-cell signaling regulates plant morphogenesis.
  • Plasmodesmata control intercellular communication by altering pore size, forming symplasmic fields.
  • Understanding symplasmic communication is key to plant cell totipotency.

Purpose of the Study:

  • Examine the role of plasmodesmata and symplasmic communication in establishing plant cell totipotency.
  • Utilize somatic embryo induction in Arabidopsis as a model system.
  • Investigate the relationship between callose deposition, symplasmic isolation, and embryo development.

Main Methods:

  • Assessed cell-to-cell communication using fluorescent tracers.
  • Performed histological and ultrastructural analysis.
  • Correlated findings with WOX2 embryo reporter gene expression and DR5 auxin response.

Main Results:

  • Embryogenic cells are symplasmically isolated from non-embryogenic cells.
  • Callose deposition in plasmodesmata precedes WOX2 expression and reduces auxin response.
  • Inhibiting callose biosynthesis suppressed somatic embryo formation.

Conclusions:

  • Callose deposition at plasmodesmata is essential for symplasmic isolation.
  • Symplasmic isolation mediated by callose is required for establishing cell totipotency in Arabidopsis.
  • Plasmodesmata regulation is a critical mechanism for controlling plant development.